Here’s a possible Top 10 levels list from More YouTube's 20th Anniversary based on their iconic status, humor, and creativity:

Ugandan Knuckles – This meme, originating from VRChat, became a huge viral sensation. The distorted, humorous version of Knuckles from Sonic the Hedgehog asking "Do you know the way?" has remained a part of meme culture for years.

Shrek/Dab – Shrek has long been a meme icon, and combining it with the dab dance move made for even more hilarious results. Shrek’s absurdity combined with the dab’s popularity makes this a memorable meme.

Michael Rosen – Michael Rosen's unique way of delivering his comedic poems and skits, especially with his signature "Chocolate Cake" video, has become a meme staple. His distinct voice and expressions are widely used for remixing and humor.

Never Say No to Panda – This meme, where the panda stubbornly refuses to be denied or challenged, has become a staple of internet humor due to its repetitive and stubborn nature. The "Never Say No to Panda" phrase is often associated with humorous fail videos.

Who's that Pokémon/John Cena – A popular meme that combines the Pokémon segment of revealing a silhouette of a Pokémon with the dramatic reveal of "John Cena!" This became a running joke, especially with the iconic You can’t see me catchphrase from John Cena.

Cocomelon Sucks! – This meme pokes fun at the children's show Cocomelon, which became the most-watched YouTube channel, with people humorously expressing their annoyance or exaggerating dislike for it.

Screaming Cat/Screaming Marmot – This meme, which involves animals screaming in a humorous or exaggerated way, became extremely popular on the internet, especially with its use in reaction videos.

Bart Hits Homer With a Chair – An iconic scene from The Simpsons that was turned into a meme due to its comedic violence and exaggerated expressions, becoming a staple in meme culture for reaction shots.

The Boiled One – A meme referring to something "boiled," either in the context of food or absurdly exaggerated situations. This meme has seen countless remixes and variations, often resulting in over-the-top humor.

Alan, Alan, Alan – This meme originates from a viral internet song where a person repeatedly sings "Alan" in a humorous, nonsensical manner. The song has been remixed countless times, and the catchy, repetitive nature makes it a fan favorite.